摘要
The human rectal adenocarcinoma cell line(HR8348)was treated withlympholdne activated killer(LAK)cells in vitro and the changes of the microtubulin inboth the effector and target cells were investigated with the aid of immunofluorescencemicroscopy.It was revealed that after the attachment of LAK cells to the tumor cells,theeffector-target conjugates formed and distribution of the microtubulin in both the effectorand target cells changed.In the LAK cells,the microtubulin concentrated mainly in thecontact region,forming a crescent-like structure,while in the target cells,themicrotubulin condensed into patches and fused with the crescent-like structure of the LAKcells,Eventually,the target cells degenerated and died.It was suggested that the lysis ofthe target cells may be related to the redistribution of the microtubulin in both theeffector and target cells.
The human rectal adenocarcinoma cell line(HR8348)was treated with lympholdne activated killer(LAK)cells in vitro and the changes of the microtubulin in both the effector and target cells were investigated with the aid of immunofluorescence microscopy.It was revealed that after the attachment of LAK cells to the tumor cells,the effector-target conjugates formed and distribution of the microtubulin in both the effector and target cells changed.In the LAK cells,the microtubulin concentrated mainly in the contact region,forming a crescent-like structure,while in the target cells,the microtubulin condensed into patches and fused with the crescent-like structure of the LAK cells,Eventually,the target cells degenerated and died.It was suggested that the lysis of the target cells may be related to the redistribution of the microtubulin in both the effector and target cells.
