细胞工程在烟草品种改良上的应用

The Application of Cell Engineering to the Variety Improvement in Tobacco

本文在扼要回顾烟草细胞工程发展的基础上,讨论了它在烟草品种改良上的作用。其中主要回顾了花培育种、细胞培养诱变与抗性筛选、原生质体融合培养技术,转移外源基因创造培育烟草变异新类型的应用原理及其近20年来所取得的主要成就。

After briefly reviewing the history of the development of tobacco cell engineering, we discussed the potential that the cell engineering may have on the variety improvement in tobacco. In this paper, we mainly reviewed monoploid breeding, mutant induction and resistant screening at the cell level, protoplast culture and fusion and etc. We also reviewed the practical foundations of these techniques in transferring alien genes to produce new to- bacco varieties. Finally, we reviewed the main achievements got in the past 20 years.