卡介苗对枯否细胞生物特性影响的免疫细胞化学研究

INFLUENCE OF BCG ON THE CHARACTERISTICS OF KUPFFER CELL OF RATS—AN IMMUNOCYTOCHEMICAL STUDY

卡介苗(BCG)是增强肝枯否细胞(KC)抗肝癌细胞作用的最佳免疫制剂之一。本实验是给大鼠BCG后,应用溶菌酶免疫细胞化学法显示KC,以图象分析系统测定KC的数量、分布和面积,以细胞分光光度计测定KC溶菌酶活性;分离KC体外受BCG作用后,用ELISA法测定RC的Fc受体表达。结果显示:BCG使KC数量增多一倍,尤以肝小叶周边带为显著;KC体积明显增大,细胞面积增大55.71％,周边带KC面积增大一倍上;细胞溶菌酶光密度增大64.35％;Fc受体表达增强64.82％。上述结果是BCG增强KC抗肝癌细胞作用有关细胞学机制。

BCG is one of the best immune stimulants which can enhance anti—hepatoma effects of Kupffer cells (KC). In this study, the rats were given BCG and the liver sections were stained by PAP immunohistochemical method of lysozyme. The number and area of KC were measured by using image analyser, and lysozyme activity was estimated by microspectrotometer. KC of normal rats were isolated and treated by BCG in vitro, then the activity of Fc receptors of KC was detected by using ELISA technique. The results showed that: (1) KC number increased up to 2 times that of the controls, especially in peripheral zone of hepatic lobule; (2) The mean area of KC was enlarged 55.71％ compared with controls, the area of KC in peripheral zone increased more than twice; (3) The mean O. D. value of lysozyme content in a single KC was 64.35％ higher than that of the controls; (4) The expression of Fc receptors of KC was enhanced 64.82％. It may be concluded that the above mentioned changes of KC induced by BCG are related to the enhancement of their anti—hepatoma effects.