电针颧髎穴和尾部电流致痛后c—fos在大鼠中枢脑桥延髓内DBH阳性神经元中的表达

CO—LOCALIZATION OF FOS—LIKE PROTEIN AND NA IN PONS AND MEDULLA NEURONS FOLLOWING ELECTRIC ACUPUNCTURE OF "QUAN—LIAO" AND ELECTRIC STIMULATION OF THE RAT TAIL

本研究在大鼠上分别用电针大鼠面部颧髎穴和尾部电流刺激致痛后,报道DBH阳性神经元与c—fos蛋白在中枢神经系统中的共存。实验在雄性SD大鼠上进行。刺激后2小时,经心脏用4％多聚甲醛磷酸缓冲液灌注固定,脑组织作冰冻冠状切片。在同一切片上顺序进行Fos抗体—ABC—DABni和DBH抗体—PAP—DAB双免疫细胞化学方法染色,并以不刺激动物作对照,结果表明:针组和痛组动物均可在脑干若干核团内观察到Fos样免疫阳性(FLI)显示神经元在细胞核上,星紫黑色。光镜下在A_1、A_2、A_3蓝斑与蓝斑下核处有DBH样免疫阳性(DLI)在细胞体、突起和终末上,呈棕黄色,但胞核不染。光镜下出现三种形式神经元:1.仅有FLI胞核染色。2.仅有DLI胞质、突起和终末染色。3.FLI胞核存在DLI胞体中(即共存)。统计分析表明。在脑干部位,FLI在网状外侧核(RL)、孤束核(nts)、延髓头端腹侧区(RVM)和导水管周围灰质的腹侧、腹外侧和外侧部位(PAG)针组显著多于痛组。共存神经元仅在A_1部位针组显著地多于痛组,A_2、A_5及蓝斑部位。针组与痛组的共存数差异不显著。

Fos, a nuclear Peptide which is the expression product of proto—oncogene c—fos, can be inducible following environmental changes and may function as an activator of gene expression. In present experiments electroacupuncture of 'Quan—Liao' and electrostimulation of the rat tail in conscious SD rats are utilized for inducing c—fos expression. Combined Fos—ABC—DAB with NA—PAP—DAB, a double immunocytochemical method, the colocalization was found in nucleus tractus solitarius (nts), nucleus reticularis lateralis (rl), locus coeruleus and the A5 region. Fos like immunoreactive(FLI) cell nuclei appeared as deep blue particles in round or oval shap. The cell bodies and processes of the NA—containing neurons Were yellow—brown, but their cell nuclei were no stain. In the colocalizing cells, the yellow—brown NA cell bodies contained the deep blue FLI nuclei, imlying that c—fos, in some extent, may be related to the biosynthesis of noradrenalin. Since NA has been known to be involved in modulation of pain, this colocalization seemed to be very interesting. The number of the neurons colocalized of EA in rl was more numerous than that of the pain group. Additionally, the number of FLI nuclei of EA in rl, nts, rostro—ventralis medulla and periaqueductalgray was markedly more numerous than that of the pain group.