实验性铬酸钠中毒大鼠肝Ⅳ型胶原变化的研究

A STUDY ON THE CHANGE OF TYPE COLLAGEN IN LIVER OF RATS TREATED BY Na_2CrO_4

用抗Ⅳ型胶原单克隆抗体免疫组化法、病理组织学及图象分析对实验性铬酸钠中毒大鼠肝进行了研究。一次气管内注入0.04 mg/kg Na_2CrO_42天后肝组织即出现病理改变和Ⅳ型胶原免疫组化阳性产物增加。一次注入0.98 mg/kg Na_2CrO_4后第2～28天Ⅳ型胶原免疫组化阳性产物均较对照组增加。增加的程度与肝病变程度一致,且随着肝组织的修复而下降。结果说明铬染毒所致的Ⅳ型胶原的改变与肝的病理改变密切相关。

The change of type Ⅳ collagen in the liver of the rats was studied by using immunohis tochemical,histopathological and image analysis methods.With 0.04 mg/kg Na_2CrO_4 ad- ministered only once through intratrachea,the pathological changes and strong positive im- munostaining by anti-type Ⅳ collagen monoclonal antibody were observed in liver tissues two days later,with 0.98 mg/kg Na_2CrO_4 administered only once,the immunostaining turned to be stronger in the experimental groups than in the control group during the period from the second day to the twenty-eighth day.The intensity of the immuncstaining was in conformity with the severity of liver elamage.With the repairment of the liver tissues,the immunostaining became weaker and weaker.The results illustrated that the change of type Ⅳ collagen caused by chromium was closely related with the pathological change in liver and suggested that type Ⅳ collagen could be regardel as a nearly marker to assess the occur- rence,development and prognosis of liver damage caused by chromium of other heavy met- als.