摘要
Infection of murine bone marrow cells with retrovirus, containing the interleukin 3(IL-3) coding sequence and the newmyecine resistant gene, resulted In the ggeneration of factor-independent, G418 resistant, and Immortalized cell line E001. The established cell line has unique cytochemistry character, two marker chromosomes, and the Robertsonian translocation of chromosome 10th. The cell needs a stromal-dependent Mage before Us autonomous growth. The E001 cells secrete IL-3 which supports the growth of factor-dependent cell line 32D. The E001 cell growth is not influenced by its own conditioned medium and the cell density. These data Indicate that the autocrine stimulating mechanism may be one of the promary events In the cell transformation, white the expression of IL-3 and/or the retrovirus Insertion may respond to the phenotype of me E001 cells. The stromal layer may also be necessary In the cell transforming process.
Infection of murine bone marrow cells with retrovirus, containing the interleukin 3(IL-3) coding sequence and the newmyecine resistant gene, resulted In the ggeneration of factor-independent, G418 resistant, and Immortalized cell line E001. The established cell line has unique cytochemistry character, two marker chromosomes, and the Robertsonian translocation of chromosome 10th. The cell needs a stromal-dependent Mage before Us autonomous growth. The E001 cells secrete IL-3 which supports the growth of factor-dependent cell line 32D. The E001 cell growth is not influenced by its own conditioned medium and the cell density. These data Indicate that the autocrine stimulating mechanism may be one of the promary events In the cell transformation, white the expression of IL-3 and/or the retrovirus Insertion may respond to the phenotype of me E001 cells. The stromal layer may also be necessary In the cell transforming process.
