人骨髓成纤维样细胞克隆亚系(HFCL-1)分泌因子的初步提纯对白血病细胞系HL60的抑制作用

INHIBITORY EFFECT OF PARTIALLY PURIFIED ACTIVITY DERIVED FROM CLONED FIBROBLAST CELL SUBLINE(HFCL-1) OF HUMAN BONE MARROW ON LEUKEMIA CELL LINE HL60

人骨髓基质成纤维细胞系及其亚克隆细胞系分泌抑制HL60细胞生长的物质。其条件培养液中的抑制物通过离子交换层析、凝胶过滤层析被部分纯化。纯化物分子量约为30000,这些物质对HL60细胞生长显示特异性抑制,而对正常骨髓中的粒系祖细胞的生长只出现轻度抑制。

The conditioned medium of human marrow fibroblast cell line was partially purified by DEAE cel-lulose-52 and Sephacryl S-300. The molecular weight of the product was around 30000 daltons. Results indicated that the active factor inhibited HL60 cell growth in both liquid and semisolid culture system but was much less effective on normal marrow CFU-GM growth.