成人急性髓性白血病的反常免疫表型

ABERRATION OF ANTIGEN EXPRESSION IN ADULT ACUTE MYELOCYTIC LEUKEMIAS

对58例未经治疗的成人AML骨髓细胞用FCM及13种MoAB进行免疫表型测定。53.4%患者骨髓单个核细胞呈现纯髓系抗原表达(Ly^-AML)。34.5%患者的骨髓细胞除髓系外伴有淋巴系抗原表达(Ly^+AML)。在Ly^+FKAML,其淋巴系抗原表达的荧光强度明显弱于其髓系抗原的。在同样的诱导缓解治疗方案下,Ly^+AML组达完全缓解天数明显长于Ly^-AML组。AML细胞这种免疫表型特点是监测残存白血病细胞的良好标志,亦对临床提示应采用相应的治疗方案。

Marrows from 58 cases of newly diagnosed acute myelocytic leukemia(FAB-AML) were immunophenotyped by flow cytometry with 13 kinds of monoclonal antibodies. 53.4% of patients marrows showed pure myeloid antigen expression (Ly AML) and 34.5%displayed both myeloid and lymphocyte associated antigen expression (Ly+AML). In genenal, fluorescence intensity of lymphocyte associated antigen in Ly+AML was weaker than that of the myeloid antigen. Cells with myeloid and lymphoid marker distributed randomly in DNA-aneuploidy and diploidy. Days to reach the first complete remission were longer in Ly+AML. The aberration antigen expression in AML allows a sensitive detection of minimal residual leukemic cells in complete remissive bone marrow and directs the treatment protocal.