摘要
Hepatitis A virus (HAV) is very persistent in the environment and is especially difficult to detect in the seafoods. In recent years, molecular cloning of the genome of HAV has led to sensitive polymerase chain reaction (PCR) method for the detection of HAV RNA. Here we describe a new and simple procedure to extract RNA from contaminated clams and the PCR method for detection of HAV in environmental samples. The specificity and efficiency of PCR amplification were studied using cDNA and RNA of HAV. Three primer couples gave satisfactory results. Some basic parameters of the PCR were modified to perform a highly specific and sensitive test.
Hepatitis A virus (HAV) is very persistent in the environment and is especially difficult to detect in the seafoods. In recent years, molecular cloning of the genome of HAV has led to sensitive polymerase chain reaction (PCR) method for the detection of HAV RNA. Here we describe a new and simple procedure to extract RNA from contaminated clams and the PCR method for detection of HAV in environmental samples. The specificity and efficiency of PCR amplification were studied using cDNA and RNA of HAV. Three primer couples gave satisfactory results. Some basic parameters of the PCR were modified to perform a highly specific and sensitive test.
