摘要
Using α-and β-tubulin cDNA Probes and Northern blot hybridization technique,we ana-lyzed the effect of intraperitoneally injected Qingyangshen (QYS),a traditional Chinesemedicine with antiepileptic property,and diphenylhydantoin sodium (DPH) on hippocampalα-and β-tubulin gene expression during kainic acid (KA) induced chronic seizures.It wasfound that:1) thirty days after intraperitoneal injection of KA,α-and β-tubulin mRNAs inanimals showing chronic seizures increased 3.02±1.05 and 4.07±1.32 times respectivelycompared with control;2) neither QYS (15 mg/kg,q.o.d.×6) nor DPH (50 mg/kg,q.o.d.×6),when used separately,could inhibit the above mentioned effect of KA;3) whenQYS (15 mg/kg,q.o.d.×6) and DPH (50 mg/kg,q.o.d.×6) were given in combination,the long-term increase in tubuliu gene expression induced by KA was significantly reduced,with α-and β-tubulin mRNAs being decreased to 0.44±0.08 and 0.50×0.10 times of corresponding values in animals treated with KA alone.The results indicate that the mechanismof antiepileptic effect of QYS is at least partially related to the inhibition of tubulin synthe-sis and subsequent reduction in mossy fiber sprouting and neosynaptogensis.
Using α-and β-tubulin cDNA Probes and Northern blot hybridization technique,we ana- lyzed the effect of intraperitoneally injected Qingyangshen (QYS),a traditional Chinese medicine with antiepileptic property,and diphenylhydantoin sodium (DPH) on hippocampal α-and β-tubulin gene expression during kainic acid (KA) induced chronic seizures.It was found that:1) thirty days after intraperitoneal injection of KA,α-and β-tubulin mRNAs in animals showing chronic seizures increased 3.02±1.05 and 4.07±1.32 times respectively compared with control;2) neither QYS (15 mg/kg,q.o.d.×6) nor DPH (50 mg/kg, q.o.d.×6),when used separately,could inhibit the above mentioned effect of KA;3) when QYS (15 mg/kg,q.o.d.×6) and DPH (50 mg/kg,q.o.d.×6) were given in combination, the long-term increase in tubuliu gene expression induced by KA was significantly reduced, with α-and β-tubulin mRNAs being decreased to 0.44±0.08 and 0.50×0.10 times of corre sponding values in animals treated with KA alone.The results indicate that the mechanism of antiepileptic effect of QYS is at least partially related to the inhibition of tubulin synthe- sis and subsequent reduction in mossy fiber sprouting and neosynaptogen(?)sis.
