巨细胞病毒感染绒毛组织后的形态学观察

Morphological Observation on Chorionic Tissue after Cvtomegalovirus Infection.

应用组织免疫酶的方法对608例早期妊娠绒毛组织行组织学连续切片。用PAP染色法染色,光学显微镜观察,以绒毛组织的细胞核、细胞质颗粒的变化确定被细胞病毒感染的绒毛组织。为验证此方法的可靠性,从已被确定为阳性感染的222例标本中,随机选取10例标本,从已确定为阴性的386例标本中,随机选取6例标本,将其低温保存的绒毛组织做电镜标本。在电镜下观察,结果发现,PAP染色阳性的组织在电镜下均发现了巨细胞病毒包涵体或病毒颗粒,其绒毛组织受到不同程度的侵害,PAP染色阴性的绒毛组织电镜下均未发现巨细胞病毒包涵体或病毒颗粒,其绒毛组织结构良好。

The histological successive sections of chorionic villiissue from 608 cases of early pregnancy were prepared microscopic observation, immunoenzyme method and PAP staining method, to identify the particle changes inne eyplasm and nuclei of the chorionic villi tissue after Cn egalovirus infection. In order to test and verify theab ihty of this method 10 samples from 222 positive ses a 'samples from 386 negative cases were selected tan and were examined with transmission electron microscope. The results indicated that virus particles(Virion) and inclusion bodies were found in the chorionic villi samples of the positive cases with PAP staining method, but have not been found in the samples of the negative cases.