LAK细胞免疫活性研究

STUDIES OF IMMUNOLOGICAL ACTIVITY OF LAK CELL

本文用^(51)Cr-铬酸钠释放法分析了由PBL、SPC和THC制备的LAK细胞免疫活性变化规律。证明LAK细胞的NK活性和LAK活性与IL-2有非常明显的正向依赖关系,与培养细胞密度有明显的负向依赖关系。无IL-2诱导,不表现LAK活性,在低细胞密度条件下少量的IL-2即可激活LAK细胞,细胞密度增大,IL-2剂量需要相应增加,用2×10~6/ml细胞密度制备LAK细胞,诱导LAK细胞活性的最佳IL-2剂量为1000 IU/ml。单用IL-2激活LAK细胞,NK活性高峰时相在48h左右;LAK活性高峰时相在60h前后。LAK细胞杀伤活性可以在培养液中维持1～2天时间,要在较长时间内维持LAK细胞活性,须定期更换培养基,添加IL-2。LAK细胞杀伤活性与细胞形态学变化不完全同步。

In the paper,the alterative regularities of the killing activity of LAK cell prepared with PBL,SPC and THC was studied by ^(51)Cr-release way. The results showed that there is apparent positive dependent relationship between the killing activity of LAK cell and IL-2 dosage,and negative dependent relationship between the killing activity of LAK cell and the cell density in culture. In the absence of IL-2 in medium,the LAK activity of LAK cell could not be determined. When the LAK cell was prepared with cell density of 2×10~6,the optimum of IL-2 was l000U/ml in medium. The peak time of NK activity appears about 48 hour and the peak time of LAK activity appears about 60 hour. The killing activity of LAK cell could maintain for 1～2 days,in order to maintain for a long,lL-2 must be added into culture medium at regular intervals. The killing activit of AK cell was not synchronous with their morphological alteration.