原发性肺癌核仁组成区定量研究

THE QUANTITATIVE STUDY OF AgNORs IN PRIMARY CARCINOMA OF LUNG

应用核仁组成区相关嗜银蛋白(AgNORs)染色技术对10例肺部良性病变和29例肺癌(12例鳞癌、9例腺癌和8例小细胞肺癌)进行核仁组成区定量研究,结果表明:肺癌各组平均每核含AgNORs量均显著高于肺部良性病变组(P<0.01),在肺癌各组中,小细胞肺癌平均每核含AgNORs量显著高于鳞癌(P<0.05)和腺癌(P<0.01),鳞癌平均每核含AgNORs量显著高于腺癌(P<0.01)。此外,AgNORs在不同病变细胞核中有一定的分布形式,恶性肿瘤中,AgNORs以聚合型和分散型为主,良性病变中,AgNORs以规则型为主。提示AgNORs定量研究有助于肺部良、恶性病变以及不同病理类型肺癌的鉴别。

Using a silver staining technique, nucleolar organizer region associated proteins (AgNORs) were studied in paraffin sections of 10 specimen ts benign lung disease and 29 lung cancers (12 squamous carcinomas,9 adenocarcinomas and 8 small cell lung cancers). The results showed that AgNORs per nucleus in the lung cancers were much more than the benign lung disease (P<0. 01), AgNORs per nucleus in small cell lung cancer were much more than those in squamous carcinoma (P < 0. 05) and adenocarcinoma (P<0. 01),AgNORs per nucleus in aquamous carcioma were much more than those in adenocarcinoma (P<0. 01). The distribution of AgNORs in nuclei of malignant and benign lung disease was different. It is suggested that the AgNORs staining is a useful technique for the diagnosis of lung cancer.