锌诱导金属硫蛋白合成预防庆大霉素肾毒性作用的体外实验

Zinc-induced metallothionein synthesis could protect nephrotoxicity from gentamicin in suspended proximal tubules of rats

本实验以直接分离大鼠近端肾小管(PT),在体外观察锌诱导金属硫蛋白合成是否能预防庆大霉素(GM)对其DNA合成、影响Na^+-K^+ATP酶的活性,以及自由基产生的状态.结果示预先注射锌后,大鼠PT能减轻GM对DNA合成和Na^+-K^+ATP酶活性的抑制,部分可能与其锌诱导金属硫蛋白清除GM产生自由基有关。

The present project was designed to study whether preinjection of zinc could protect from the gentamicin harmful effects on DNA synthesis,Na^+-K^+ATPase activity and free radicals production in rats' renal proximal tubules (PT) in vitro. The results showed DNA synthesis and Na^+-K^+ATPase activity were not suppressed by gentamicin in Zn-preinjected rats' PT, and the metallothionein in Zn-preinjected rats' PT was very significantly higher than that in the normal and saline preinjected rats' PT(P<0.001). Our data indicated that preinjection of zinc could ameliorate gentamicin-induced nephrotoxicity via the induction of the metallothionein synthesis in rats' PT to scavenge free radicals generated by gentamicin.