ELISA法检测γ-干扰素

The Study on interferon-gamma by a sandwich enzyme immunoassay

提要用高纯度重组人IFN-Y免疫家兔,制成抗IFN-Y多克隆抗体,建立了双抗体夹心ELISA法。本法最小检出量为1.56IU/ml,特异性好,不精密度批内CV4.6％～7.2％,批间CV6.9％～10.1％,回收率96.7％～105.3％。30名健康成人外周血细胞经PHA诱导后上清中IFN-Y活性为7.1士4.2IU/ml。

Human interferon-gamma was detected by a sandwich enzyme immunoassay using an antirecombinant interferon-gamma polyclonal antibody. The assay was specific and could detect levels of IFN-as low as 1. 56 IU/ml. The intraassay and interassay coefficients were 4. 6～7.2% and 6. 9～10. 1% respectively. The analytical recoveries were 99. 1～105. 3% . Thirty normal aludts were determined for IFN-in supernatants of PHA-activated blood cell and the results was 7. 1±4. 21 IU/ml.