兔出血症出血机理的研究

A STUDY ON HAEMORRHAGIC MECHANISM IN RABBIT HEMORRHAGIC DISEASE

对出血症病兔的骨髓及血小板用光镜、透射和扫描电镜及免疫组织化学方法进行了研究。结果表明,本病的主要病变为骨髓巨核细胞发生退行性变化。电镜下,巨核细胞的胞浆界膜管道系统内出现病毒颗粒,血小板异常并出现巨血小极,血小板呈球拍状,花瓣状或蜂窝状膜复合体结构。扫描电镜下,血小板呈镰刀形,有时几乎呈环形。免疫组织化学染色,在骨髓巨核细胞浆内观察到黑色的胶体金颗粒。作者认为,兔出血症的出血机理主要是与骨髓巨核细胞受损伤有关,导致血小板生成障碍而数目减少,从而引起全身各组织器官的出血。

Bone marrow and platelets from rabbits with hemorrhagic disease(RHD)were investigated by lightmicroscopy,transmission electronmicroscopy,scanning clectronmicroscopy and immunolgold-silver staining(IGSS).The main appearence was regressive changes of bone marrow megalocaryoeytes.Under electronmicroseope,there werevirions in cytoplasm limiting membrane tubes of megalocaryocytes.The platelets were abnormal,becoming hattledorelike,petaloid or honeycomb membrane complex structures.When observed under scanning electronmicroscope,theywere sickle,sometimes ring in shape.The cytoplasm of megalocaryocytes was showed positive reaction by IGSS.Theauthors suggested that the hemorrhagic mechanism of RHD was related to lesions of megalocaryoeytes,resulting inplatelets dyspoiesis and then reduction,and finally inducing systemic hemorrhage of different organs.