体外实验——湿润烧伤膏对家兔皮肤活性的影响

THE EFFECT OF MEBO ON RABBIT SKIN VITALITY

本实验采用测定家兔皮肤内琥珀酸脱氢酶(SDH)的方法,测定了不同大小皮块以及与湿润烧伤膏(MEBO)不同混合浓度时的皮肤SDH值,作为实验组;测定置于常规皮肤保存液中的小皮块SDH值,作为对照组。两组结果表明,不同大小皮块的对照组与实验组之间,其活性百分值曲线是相同变化趋势,而且活性百分值与混合浓度大体呈正相关。这表明湿润烧伤膏对皮肤SDH值无明显影响。

In this experiment, the activity of succinic acid dehydrogenase (SDH) in rabbit skin was determined to reflect the vitality of the skin. Full-thickness of the skin from rabbit back was taken. The skin was cut into cubes, 5 mm3or 10 mm3 in size. In the experiment group, the skin cubes were mixed with different concentrations of MEBO. 8, 15, 30 or 50 skin cubes in one gram of MEBO. In the control group, the skin cubes were stored in normal saline solution. The skin cubes of the two groups were stored in tightly closed container at a temperature of 20 ℃ The SDH activity of the freshly prepared skin cubes and the activity on 1, 2, 4 and 7 days after storage were determined respectively in the experiment and the control groups. The results were shown by curves on coordinate paper as the percentage of skin vitality. The results revealed that 5mm3and 10mm3skin cubes of the control and the experiment groups had their vitality percentage curves changed with the same tendency. This implied that MEBO had no significant effect of rabbit skin vitality. Vitality percentage and the mixing concentration were almost positively correlated. The more the MEBO was adhered to the skin cubes, the more serious the hypoxia of the skin and the more serious the loss of vitality occurred.