外源遗传物质诱导的家蚕变异品系酯酶同工酶比较

Study on Blood Esterase from the New Variant Strains of Bombyx mori L.Induced by Exogenous Genetic Substance

采用不连续聚丙烯酰胺凝胶电泳法,对由蓖麻蚕DNA导入家蚕和蓖麻蚕()与家蚕()杂交获得的变异品系,进行了血液酯酶同工酶酶谱的比较分析,与受体或母本比较,变异品系的酯酶同工酶酶谱中,或者出现某些新的酶带,或者缺失了某些原有的酶带,但是没有出现供体或父本所特有的酶带.

The isozymograms of the blood esterase from the variant strains, which bred from both introduction of eri silkworm DNA into domesticated silkworm and hybridization of eri silkworm (♂) and domesticated silkworm (♀) ,were analyzed and compared by discontinuous PAGE. Being compared with those of the receptor or the female parent,the esterase isozymo grams of the variant strains either present some new bands or absent some bands that the parents originally have. But, there are not any characteristic bands of the donor or the male parent in the esterasc isozymograms of the variant strains.