摘要
取5μl溶解在20mmol/L磷酸缓冲液(pH7.0)中的烟草花叶病毒(0.2mg/ml),将其滴加在新鲜剥离的云母表面上,然后用0.5％的磷钨酸染色。所得样品在大气下用原子力显微镜进行成像,用AFM可以清楚地观察到棒状的TMV拓扑结构,用磷钨酸染色后的TMV样品能稳定地吸附在新鲜剥离的云母表面上,可靠的TMV的AFM像在大气下可容易地获得。
Tobacco mosaic virus (TMV) suspended in 20 mmol / L phosphate buffer, pH 7, at concentration of 0.2 mg/ml was deposited on freshly cleaved mica surface, then stained with 0.5% buffered phosphotunstic acid solution. The TMV samples treated by the above procedures were imaged with AFM in air at room temperature. The structure of TMV imaged with AFM were analyzed and discussed.
基金
国家自然科学基金
中国科学院基金
