摘要
DNA中胞嘧啶甲基化及其在基因调控中的作用,是一个很有兴趣的生物学问题。本文以d(CGCGAATTCGCG)为对象用分子模建、能量优化、均方根偏离分析等方法,从理论上探讨了胞嘧啶甲基化对DNA精细结构起伏的影响,证实了甲基化作用主要引起5-甲基胞嘧啶所在局部位置的DNA精细结构发生变化,而糖链部分发生的变化远大于碱基部分发生的变化。
Model building, energy minimization and root-mean-square analysis were applied to study the effects of cytosine methylation of d(CGCGAATTCGCG) on DNA fine structure, The results showed that the structure fluctuations in phosphate backbone were greater than those in base pairs, and that the variations in phosphate backbone, base pairs as well as in the whole DNA structure mainly appeared in d(CGCG) segment where the cytosine methylations had happened.
关键词
甲基化
分子力学
均方根偏离
DNA精细结构
Methylation, Molecular mechanic, Root-mean-square deviation, DNA fine structure
