Ⅰ型单纯疱疹病毒与其同属病毒及寄生宿主核酸同源性的初步研究

Preliminary Study on the DNA H mology of HSV-1 with its Same Genus Viruses and Parasitic Host

直接从基因定位角度出发,用斑点核酸杂交法初步探讨了HSV-1(1SM_(44)株)DNA EcoR IC、F两个片段与其同属病毒及寄生宿主DNA的同源性。结果提示:人染色体DNA中含有与C片段互补的同源序列;F片段为HSV特异的型共同性片段。本文为致病病毒和宿主之间相互作用的分子基础研究以及HSV基因探针的制备奠定了基础。

From the aspect of gene localizatior, preliminary study was performed on the DNA Homology of HSV-I(SM44 stain) EooR J C, F fragments with its same genus viruses and parasitic host. The results showed that human chromosomal DNA has homologous sequence complimentary to the C fragment, and F fragment is HSV specific fragment. This article provided some basis for basic molecular study of the interaction between pathogenic virus and the host, and also for the preparation of HSV gene probe.