还原抗肺腺癌单抗^(99m)Tc直接标记的实验研究

EXPERIMENTAL STUDY OF DIRECT TECHNETIUM-99m LABELING OF REDUCTION-MEDIATED MONOCLONAL ANTIEODY OF LUNG ADENOCAREINOMA

报告用自行制备的抗人肺腺癌单抗CMU—15A进行^(99m)Tc直接标记及荷瘤裸鼠体内分布的研究。该单抗经免疫学组织化学等证实对肺腺癌有较高的特异性。采用还原抗体的方法,直接用^(99m)Tc标记,标记率在95%以上,免疫活性检测证明标记后抗体活性保持良好。体内分布实验表明,经尾静脉注射标记抗体16h后,T/NT值较高,并明显高于对照组,提示标记单抗在肿瘤组织中出现了较好的特异性浓聚。

Method of direct labeling monoclonal antibody(McAb) CMU 15A of human adenocarcinoma of lung with Tc-99m and study of its distribution were reported.The rate of labeling was high (>95%). Distribution of the McAb in nude mice grafted with human lung adenocarcinoma showed that after 16 hours of injection, T/NT values were higher and significantly higher than those of controls, indicating that McAb CMU 15A had a high and specific uptake in the tumor. The results showed that the labeling method was quick, simple, higher rate of labeling, less affect on McAb ard ideal uptake into tumor, which will be beneficial for its application in indicinmunoimaging.