南昌链霉菌(Streptomyces nanchangensis)固体种子培养的研究

STUDIES ON SCREENING THE MOST FAVOURABLE SOLID SEED-MEDIUM AND CULTURAL CONDITIONS FOR STREPTO MYCES NANCHANGENSIS

本研究进行了南昌链霉菌孢子培养基和培养条件的筛选.筛选到的培养基和培养条件,使南昌链霉菌每支斜面孢子达5亿多,是高氏培养基产孢量的7.5倍,培养时间缩短了7～10d.同时初步探讨了南昌链霉菌在固体培养基上孢子的消长变化及对摇瓶发酵产素的影响,发现该菌在固体培养基上能周期性地产生多代孢子;摇瓶发酵,接种第一代孢子南昌霉素 A 产量最多,接种第二代孢子则高效杀虫组分 B 的效价最高.

The most favourable solid seed-medium and cultural conditions were scree ned out through orthogo-nal experiments.Under such suitable conditions the number of spores produced was increased 7.5 times morethan those on Cause's No.1 synthetic medium while the culture time needed only 4 days,i.e.being shortenedby at least 7-10 days.The curve of the spore number produced at different times showed that there were several generationsof spores produced on the same agar-slants;each generation's spores possessed different characteristics inantibiotical production.The fermentation flasks inoculated with the first or the second generational sporeswould produce more nanchangmyein A.or more effective insecticidal antibiotics (The fraction B) respectively.