幽门螺旋菌在双相培养基中生长特性的研究

Biphasic Culture System for Study of Microbiol Growth of Helibacter Pylori

本文观察了幽门螺旋菌在固体及液体—固体双相培养基上生长的特性,37℃72小时培养后,定量实验表明,固体培养基上菌数为1.02×10~2cfu/ml,而双相培养基上则为1.14×10~4cfu/ml;用血液双相培养基,对CP进行了生长曲线的测定,从0小时加入1.02×10~2cfu/ml开始,按每天平皿活菌计数,共5天(24、48、72、96、120、144h)分别为2.5×10~2cfu/ml,4.97×10~2cfu/ml,1.14×10~4cfu/ml,1.24×10~5cfu/ml,2.87×10~5cfu/ml,9.75×10~4cfu/ml以后逐日下降;液相培养液24—144小时pH测定,pH由7.2下降至6.4—6.9。

The results reported herein are the growth rate and characteristics of Helicobacter pylori cultured with biphasic(Solid-Broth) Brucella agar supplemented with 6％ sheep blood, and the brucella broth with an additional of 10％ fetal bovine serum. Satisfactory growth of Helicobacter Pylori 89404 was obtained with biphasic Brucella blood agar. The number of viable cells was 1.14×10~4 cfu/ml after 72h incubation at 37℃ compared with 1.02×10~2 cfu/ml cultured on the solid blood medium and incubated under the same condition. HP(Helicobacter pylori)89404 grew on the biphasic blood medium mentioned above survived up to 6 days, the pH of the broth was changing from 7.2 to 6.4.