肺心病患者血浆组织型纤溶酶原激活物含量与活性变化的意义

Significance of changes in plasma levels of tissue plasminogen activator in patients with chronic cor pulmonale

用酶联免疫吸附法和发色底物分解显色法测定15例健康人和20例肺心病患者血浆组织型纤溶酶原激活物(t—PA)含量和活性,结果表明:肺心病急性期与缓解期患者血浆 t—PA 含量均显著高于健康人(P<0.001);肺心病急性期患者血浆 t—PA 活性明显低于健康人(P<0.001),缓解期则与健康人无明显差异(P>0.05);血浆 t—PA 含量和活性与血浆纤维蛋白原含量之间无相关。对肺心病患者血浆 t—PA 含量与活性变化的意义进行了探讨。

The plasma levels and activity of tissue plasminogen activator (t-PA) were determined in 15 normal subjects and 20 patients with chronic cor polmonale (CCP) by the enzyme linked immunosorbent assay (ELISA) and the spectrophotometric assays with chromogenic sub strates.It was found that the levels of t-PA antigen in patients with cor pulmonale (in both acute and remission stage) were markedly higher than those of normal subjects (P 0.001),while the levels of t-PA activity during acute at- tact of CCP were markedly lower than those in normal subjects (P<0.001).No significant difference was found between the levels of t-PA during the remission stage of CCP and those in normal subjects (P>0.05). Plasma level of t-PA antigen and t-PA activity did not correlate with fibrinogen level.The significance of changes in plasma levels of t-PA antigen and t-PA activi- ty was discussed.