MNNG诱导离体培养人类胃粘膜上皮细胞微核及畸形核的形成

FORMATION OF MICRONUCLEUS(MNs)AND ABERRANT NUCLEUS (ANs) OF HUMAN GASTRIC EPITHELIAL(HGE)CELLS INDUCED BY MNNG IN VITRO

我们使用原代培养的人胃粘膜上皮细胞,经MNNG处理诱导出微核及畸形核的形成。人胃粘膜上皮细胞来源于胃癌或溃疡病人大部切除胃的远端非病变粘膜,经酶学消化生长在含1％血清及胰岛素、转铁蛋白,EGF、牛脑垂体提取物等添加剂的DME/F12培养基中。MNNG处理24小时。不同个体对MNNG的毒性作用的反应差别很大。有的细胞在10^(-5)M仍继续生长,有的则在10^(-6)时就大量脱落死亡。五例个体均诱发出了微核及畸形核的形成。未经处理的细胞微核形成率是0,但有极小量的畸形核(0.25～8‰),MNNG在10^(-4)-10^(-7)M范围内约可诱发微核形成,同时使畸形核的出现率大大增加。这一结果首次以体外培养人胃上皮为靶细胞,显示了MNNG诱导的细胞核改变,可能为胃癌的化学病因提供更直接的证据。

Primary adult HGE cells were plated on to 24 well clusters or plastic slides in DME/F12 medium supplemented with l% serum and other growth factors. The cells were treated with MNNG at concentration of 10-4-10-7M for 24 hours. Cytotoxicity and MNs/ANs formation have been determined. The effect of MNNG varied among individuals shown by the rates of DNA synthesis. MNs could be induced by MNNG in the cells from all donors tested with a different percentage and ANs were apparently increased up to 9.6 folds. This is the first evidence that MNNG caused cytogenetic damage on human gastric epithelial cells in vitro. Our results is useful for the futher study of human stomach carcinogenesis.