APPLICATION OF DIRECTLY SEQUENCING FOR DOUBLE STRAND DNA IN RESEARCH OF ENVIRONMENTAL MUTAGENS——Ⅰ.SEQUENCING OF TC GENE FRAGMENT OF PBR322

A method of directly sequencing for double strandDNA by using Tc resistance gene of pBR322 as templateand oligonucleotide 5’-GGCCCTTTCGTCTTC-3’as primerwhich is located on the adjacent to the EcoRI sitewith the counterclockwise was presented.