摘要
应用脑立体定向方法进行兔脑缺血灶内胎脑细胞移植的实验研究。结果发现脑立体定向移植术是成功的,所有免疫组化切片上看到的血清素阳性细胞均位于脑缺血坏死灶的边缘或附近。移植神经元能够在脑缺血灶内存活、生长并成熟。电镜观察到植入神经元发出细胞突起与宿主细胞形成典型的传入或传出性突触联系。
We report here the successful performance of the stereotactic operation to transplant the fetal neurons into ischemic adult rabbit brain. Fetal serotonergic neurons of raphe nuclei were dissected free 18-20 days embryonic rabbit brain, and they were transplanted gently under stereotactic control into the brain's ischeroic focus. One, two and three months respectively after trans plantation, the brain of grafted rabbits were processed for immunohistochemical detection of 5-HT with specific 5-HTantibody. 5-HT neurons were found in the sites of brain ischemia. They exkibited the morphology of adult neurons, with well developed process (axon or dendrites) extending far fron the injection site. The ultrastructural study disclosed the existence of afferent and efferent synapses in the grafted neurons.
出处
《立体定向和功能性神经外科杂志》
1991年第2期10-12,65,共4页
Chinese Journal of Stereotactic and Functional Neurosurgery
基金
国家自然科学基金
