玉米素核苷间接酶联免疫吸附测定法的建立

DEVELOPMENT OF AN INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAYS FOR ZEATIN RIBOSIED

报道了一种利用兔抗 ZR 抗血清和辣根过氧化物酶标记的羊抗兔 IgG 建立的 ZR 固相抗原型(或称间接)酶联免疫吸附测定法;该法的线性测定范围是0.05～12.5ng;本文表明应用生物素抗生物素标记系统能大大改善本法的测定灵敏度.

This paper reported an indirect enzyme immunoassays for ZR, which was developed with rabbit antiserum against ZR-BSA and HRP-labeled goat anti-rabbit IgG.Its linear assay range was from 0.05 ng to 12.5 ng.This paper also showed that the application of avidin-biotin complex could remarkably improve the detective limit.