摘要
本研究对妊娠三个月内的胚泡植入部位蜕膜及胎盘绒毛组织的滋养层细胞进行了光镜HE、纤维素及免疫细胞化学染色观察.光镜下,植入部位的蜕膜中弥漫地浸润着一种较大的圆形、多边形或梭形的细胞,它们核的大小、形状不一,多为单核,少数为双核或多核,胞浆丰富,嗜伊红或双色,常分布在螺旋动脉周围,浸润其管璧并侵入其管腔,甚至可完全取代其管壁。免疫细胞化学观察显示这类细胞HCG及HPL染色均可为阳性,但HCG阳性率很低,并且其HCG染色明显弱于合体滋养层细胞,HPL染色显著强于合体滋养层细胞,细胞滋养层细胞的HCG、HPL染色均为阴性。β-HCG及SP-1的染色在这几种滋养层细胞中的情况与HCG一致,而PAPP-A染色无特异性。胚泡植入部位具有上述特点的细胞即为中间型滋养层细胞,现多认为这类细胞由细胞滋养层细胞演变而来,又可演变为合体滋养层细胞,是后两种滋养层细胞的移行过渡形式。
This report presents microscopic observation on morphological and immuno- cytochemical characteristics of the trophoblast cells of the human decidua at the implantation site in the first trimester pregnancy .The infiltration of a unique type of large cells different from cytotrophoblast (CT) and syncytotrophoblast (ST) was found in the decidua at the implantation site. These unique cells are termed inter- mediate trophoblast (IT). They are typically mononucleate, but may be binucleate or multinucleate. The mononucleate cells vary in shape from round, polyhedral spindle shaped. Their cytoplasm is typically abundant and eosinophilic or amphophilic. The nuclei may vary in size and shape. These cells usually distribute around the spiral arterioles, differse blood vessel wall, penetrate into the lumen, and even replace the blood vessel wall totally. Immunocytochemically, both these cells and ST are stained positive for HCG and HPL. However, the HCG-stained Cell population of IT.is much lower than that of ST,while the of HPL stained cell population of IT is significantly higher than that of ST.On the other hand,neither HCG nor HPL are positive in CT. The results of SP-1 β-HCG usually go with those of HCG.in CT, ST and IT.The PAPP-A gives non specific staining result. It is believed that IT,with its distinct morphological and immunocytochemical feats,is regarded as a transitional form in the shift from CT to ST.
出处
《解剖学报》
CAS
CSCD
北大核心
1991年第S1期20-24,134,共6页
Acta Anatomica Sinica
关键词
中间型滋养层细胞
植入部位
免疫细胞化学
Intermediate trophoblast
Implantation site
Immunocytochemistry
Human decidua