期刊文献+

人胚泡植入部位蜕膜内中间滋养层细胞光镜与免疫细胞化学观察 被引量:4

THE HISTOLOGICAL AND IMMUNOCYTOCHEMICAL OBSERVATION OF THE INTERMEDIATE TROPHOBLAST OF THE HUMAN DECIDUA AT THE IMPLANTATION SITE
下载PDF
导出
摘要 本研究对妊娠三个月内的胚泡植入部位蜕膜及胎盘绒毛组织的滋养层细胞进行了光镜HE、纤维素及免疫细胞化学染色观察.光镜下,植入部位的蜕膜中弥漫地浸润着一种较大的圆形、多边形或梭形的细胞,它们核的大小、形状不一,多为单核,少数为双核或多核,胞浆丰富,嗜伊红或双色,常分布在螺旋动脉周围,浸润其管璧并侵入其管腔,甚至可完全取代其管壁。免疫细胞化学观察显示这类细胞HCG及HPL染色均可为阳性,但HCG阳性率很低,并且其HCG染色明显弱于合体滋养层细胞,HPL染色显著强于合体滋养层细胞,细胞滋养层细胞的HCG、HPL染色均为阴性。β-HCG及SP-1的染色在这几种滋养层细胞中的情况与HCG一致,而PAPP-A染色无特异性。胚泡植入部位具有上述特点的细胞即为中间型滋养层细胞,现多认为这类细胞由细胞滋养层细胞演变而来,又可演变为合体滋养层细胞,是后两种滋养层细胞的移行过渡形式。 This report presents microscopic observation on morphological and immuno- cytochemical characteristics of the trophoblast cells of the human decidua at the implantation site in the first trimester pregnancy .The infiltration of a unique type of large cells different from cytotrophoblast (CT) and syncytotrophoblast (ST) was found in the decidua at the implantation site. These unique cells are termed inter- mediate trophoblast (IT). They are typically mononucleate, but may be binucleate or multinucleate. The mononucleate cells vary in shape from round, polyhedral spindle shaped. Their cytoplasm is typically abundant and eosinophilic or amphophilic. The nuclei may vary in size and shape. These cells usually distribute around the spiral arterioles, differse blood vessel wall, penetrate into the lumen, and even replace the blood vessel wall totally. Immunocytochemically, both these cells and ST are stained positive for HCG and HPL. However, the HCG-stained Cell population of IT.is much lower than that of ST,while the of HPL stained cell population of IT is significantly higher than that of ST.On the other hand,neither HCG nor HPL are positive in CT. The results of SP-1 β-HCG usually go with those of HCG.in CT, ST and IT.The PAPP-A gives non specific staining result. It is believed that IT,with its distinct morphological and immunocytochemical feats,is regarded as a transitional form in the shift from CT to ST.
出处 《解剖学报》 CAS CSCD 北大核心 1991年第S1期20-24,134,共6页 Acta Anatomica Sinica
关键词 中间型滋养层细胞 植入部位 免疫细胞化学 Intermediate trophoblast Implantation site Immunocytochemistry Human decidua
  • 引文网络
  • 相关文献

参考文献9

  • 1De wolf,et al.The human placental bed: Electron microscopic study of trophoblastic invasion of spiral arteries. American Journal of Obstetrics and Gynecology . 1980
  • 2Wisloki GB.The histology and cytochemistry of the basal plate and septae placentae of the normal human placenta delivered at full term. The Anatomical Record . 1951
  • 3Latta JS,et al.The differentiation of a special form of trophoblast in the human placenta. American Journal of Obstetrics and Gynecology . 1957
  • 4Dailenbach-HelIweg G,et al.Morphological and histochemical observations on trophoblast and decidua of the basal plate of the human placenta at term. American Journal of Anatomy . 1964
  • 5Kurman RJ,et al.Immunohistochemical localization of placental and chorionic gonadotropin in the normal placenta and trophoblastic tumors with emphasis on intermediate trophoblast and the placental-site trophoblastic tumor. International Journal of Gynecological Pathology . 1984
  • 6Wynn RM.Cytotrophoblastic specializations: An ultrastructural study of the human placenta. American Journal of Obstetrics and Gynecology . 1972
  • 7Angel E,et al.Immunohistochemical demonstration of placentall hormones in the diagnosis of uterine versus ectopic pregnancy. American Journal of Clinical Pathology . 1985
  • 8Terzakis JA.The ultrastructure of normal human first trimester placenta. Journal of Ultrastructure Research . 1963
  • 9Kurman RJ,et al.Intermediate trophoblast-a distinctive form of trophoblast with special morphological, biochemical and functional features. Placenta . 1984

同被引文献7

  • 1张建民,解剖学报,1991年,22卷,18页
  • 2Chan JKC, Tsang WYW. Uterina and fallopian Tubes [M]. The difficult diagnosis in surgical pathology. Philadelphia, 2003 : 465 - 533
  • 3Wan Sk,Lam PWY,Pau MY,et al. Multiclefted nuclei a helpful feature for identification of intermediate trophoblastic cells in uterine curetting specimens[J]. Am J Surg Pathol, 1992,16:1226
  • 4Silverberg SG. Surgical Pathology of the Uterus[M]. New York ,John Wiley Sons, 2002 : 198 - 199
  • 5Mutch DG. Endometrial adenocarcinoma estrogen receptor content:association of clinic pathologic features with immunohistochemical analysis compared with standard biochemical methods[J]. Am J Obstet Gynecol, 2004,157:924
  • 6张建民,麻晓迁,王建华,尹克铮.人早孕宫内膜三种滋养层细胞的鉴别[J].南京铁道医学院学报,1991,10(2):102-105. 被引量:1
  • 7张建民,许懃安,杨瑞兰,吴德明.10例胎盘部位滋养层细胞肿瘤临床、病理免疫组化及超微结构研究[J].临床与实验病理学杂志,1992,8(1):1-4. 被引量:10

引证文献4

二级引证文献5

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部