急性缺血对犬的大脑皮层锥体细胞影响的实验形态学研究

A MORPHOLOGICAL STUDY OF THE EFFECT OF ACUTE EXPERIMENTAL ISCHEMIA ON THE PYRAMIDAL CELLS OF CANINE CEREBRAL CORTEX

本研究采用成年健康家犬10只(其中正常对照组2只,实验性缺血组8只)。观察在急性缺血情况下大脑皮层(相当于额前区)锥体细胞内酶组织化学及超微结构的变化。结果表明急性缺血组(犬)的琥珀酸脱氢酶(SDH),单胺氧化酶(MAO)和三磷酸腺苷酶(Mg^(++)-ATPase)活性明显降低;乳酸脱氢酶(LDH),酸性磷酸酶(ACP)和乙酰胆碱脂酶(AchE)的活性增强.超微结构显示急性缺血组神经细胞核周间隙增宽,线粒体嵴断裂,消失和囊状化。说明急性缺血对大脑皮层神经细胞酶的组织化学及超微结构均有较明显的影响。

In the present study, 10 healthy adult dogs were used,8 for the experimental group and 2 for the normal group. We observed the histochemical and ultrastrac- tural changes of the enzymes in the pyramidal cells in the cerebral cortex under the condition of acute ischemia. The result demonstrated that the activity of LDH, AcP and AchE was increased, while the activity of SDH, MAO, ATP ase was decreased. The ultrastructural chnges showed that in the acute ischemia gromp, there were enlargement perinucleus spaces of neurone, brisement, disappearance and vasicularization of the mitochondrial crista. This indicated that acute ischemia had an obvious effect on the histochcmistry of enzymes and the ultra- structure of the neurone in the cerebral cortex.