未受刺激的大鼠脾悬液细胞的Ia抗原表达的观察

AN OBSERVATION OF EXPRESSION OF Ia-ANTIGEN ON CELLS IN SUSPENSION FROM UNSTIMULATED RAT SPLEENS

本研究采用ABC免疫酶标和间接免疫荧光技术,观察了从未受刺激大鼠脾分离的富含淋巴树状突细胞组分内细胞的Ia抗原表达.结果表明,染色标本中主要有3类细胞:1、胞体大,表面伸出树枝状突起,ABC法染色时,呈棕褐色,免疫荧光染色时,发强淡绿色荧光.2、胞体较大,呈圆形或卵圆形,无明显突起,ABC法染色时,不着色或呈淡棕黄色,免疫荧光染色时,不发荧光或发淡黄绿色荧光。3.淋巴细胞,胞体大小不一,呈球形,染色结果与2类细胞相似.文章结合SEM观察和S-100蛋白染色结果论证了这三类细胞依次为淋巴树状突细胞、巨噬细胞和淋巴细胞。结果证实淋巴树状突细胞表面持续表达大量Ia抗原。

Expression of Ia-antigen on cells in lymphoid dendritic cells (LDCs)-enrt- ched fractions isolated from unstimulated rat spleens was observed with ABC immunoperoxidase and indirect immunofluorescent staining methods. The results appeared that there were three types of cells in stained preparaions: 1. Large cells with dendritic processes on their surfaces showed dark brown in colour with ABC staining method and emitted intense light-green fluorescence with immuno- fluorescent staining technic. 2. Large rounded or ovoid cells without obviously ramifications revealed colourless or light yellow-brown in colour with ABC sta- ining method and emitted no or weak yellow-green fluorescence with immuno- fluorescent staining technic. 3. Spherical lymphocytes of varying in size were similar to type 2 cells in immunoreactivity. The results of SEM and S-100 pro- tein staining confirmed that these three types of cells were LDCs, macrophages and lymphocytes in turn. It is indicated that LDCs consistently expressed Ia- antigen in large amount on their surfaces.