RPHI和HI对EHF患者进行血清学分型的比较研究

COMPARISON OF DIFFERENTIATING SEROTYPE TO EHF PATIENT SERA WITH RPHI AND HI

作者应用RPHI和HI两种方法,对EHF患者进行血清学分型的比较。通过对57例不同病日的EHF患者血清与野鼠型及家鼠型病毒血凝素抗原(分别称为A-Ag及R-Ag)同时进行HI、RPHI。在EHF疾病过程中,两种抗体的效价呈平行性升降,存在明显的正相关(用A-Ag时,r=0.94;用R-Ag时r=0.98),对3,5,7,10,17病日的患者进行可分型率的统计发现,HI,RPHI的可分型符合率为58,75,96,36,100,100％;另对189份荧光阳性的患者血清检测的结果,HI,RPHI两法判别家、野鼠型的总符合率为90.5％。上述结果表明:与HI相比,RPHI用于EHF患者的血清学分型,不仅操作简便,勿需复杂费时的血清处理,并具有更高的特异性和灵敏度,可普遍应用于患者的早期诊断和血清流行病学的分型研究。

57 EHF patient sera of different days of illness with wild—mouse, house—rat types EHFV antigens were detected by HI, RPHL It was showed that two kinds of antibodies appeared developing parallel changes during the disease process, and expressed perfect positive correlation (r respectively was 0.94, 0.98 with A—Ag or R-Ag). In 3, 5, 7, 10,17 days of onset the differentiating correspond rate of two methods was 58, 75, 96.36, 100, 100 %. The total correspond rate of distinguishing house—rat, wild—mouse types with HI, and RPHI to 189 patient sera of IF A positive was 90.5%. The above results indicated that the RPHI was not only simple and convenient, unnecessary complicated serum treatment but also possessed with higher specificity and sensitivity in comparison with HI, and generally practicable to early diagnosis of EHF patient and serotype study of sera epidemilogy.