反向间接血凝法检测畜禽血清乙型肝炎表面抗原及其探讨

TO TEST HBsAg IN DOMESTIC ANIMAL SERA THROUGH REVERSE INDIRECT BLOOD COAGULATION

应用反向间接血凝检测畜禽血清中人类乙型肝炎表面抗原,结果各种畜禽血清均发现有阳性,且比率甚高(18.3-30.9％)。笔者试将受检血清分别经过HRBc吸收,抗HBs中和等方法处理,发现畜禽血清存在HRBc天然凝集素和与HBsAg不同的类似抗原,这些非特异性因素使血凝试验出现高比率的阳性,因此,在动物血清做反向间接血凝前,应设法排除非特异性因素、结果较为可靠。

Human HBsAg was found to be positive in domestic animal sera by rean of reveise indirect blood coagulation. The ratio is 13.3-30.9%. was also found thai domestic animal sera contained HRBc natural agbin inins and antigens different from HBsAg after the sera to be tested vr as treated through HRBe absorption and anti-HBc neutralization respectively. These nonspecific factors led to high ratio of positiveness in the blood coag-ulation test. Therefore, before reverse indirect blood coagulation nonspecific factors should be removed so as to get reliable results.