摘要
The 60% ethanolic extract from Gracilaria textorii (Rhbdophyta) was degraded with B- agarase, and certain charged (sulfated) and neutral oligosaccharides were separated by using DEAE Sephadex A 25 and Bio- gel P6 , P2 chromatographic techniques . Some of the charged oligomers were verified to be neoagarotetraose - 63- sulfate ( DP2 ). neoagarohexaose- 63 】 65 - disulfate ( DP3 ) and neoagarooctaose- 63, 65, 67-trisulfate (DP4) by using 13C-and ’H-NMR spectroscopy . One neutral oligomer was assumed to be a mixture of methylated neoagarotetraoses (DP2 ) by 1H-NMR spectroscopy .These oligomers assigned by their chemical shifts may be used as the model compounds for the structural investigation of the agar- type sulfated polysaccharides using the B- agarase degradation method .
The 60% ethanolic extract from Gracilaria textorii (Rhbdophyta) was degraded with B- agarase, and certain charged (sulfated) and neutral oligosaccharides were separated by using DEAE Sephadex A 25 and Bio- gel P6 , P2 chromatographic techniques . Some of the charged oligomers were verified to be neoagarotetraose - 63- sulfate ( DP2 ). neoagarohexaose- 63 > 65 - disulfate ( DP3 ) and neoagarooctaose- 63, 65, 67-trisulfate (DP4) by using 13C-and 'H-NMR spectroscopy . One neutral oligomer was assumed to be a mixture of methylated neoagarotetraoses (DP2 ) by 1H-NMR spectroscopy .These oligomers assigned by their chemical shifts may be used as the model compounds for the structural investigation of the agar- type sulfated polysaccharides using the B- agarase degradation method .
