摘要
Human fetal liver cells were cultured in vitro for 12h and the supernatant(Fetal liver cell conditioned medium,FLCM)was collected.The effects of FLCM ongranulopoiesis were studied.The results show that when combined with colonystimulating factor(CSF),FLCM could significantly stimulate the proliferation of normalmyctoid progenitor cells(CFU-e),and increase ~3H-TdR incorporation into bone mar-row cells.The data suggest that FLCM contains a CSF synergistic activity.
Human fetal liver cells were cultured in vitro for 12h and the supernatant (Fetal liver cell conditioned medium,FLCM)was collected.The effects of FLCM on granulopoiesis were studied.The results show that when combined with colony stimulating factor(CSF),FLCM could significantly stimulate the proliferation of normal myctoid progenitor cells(CFU-e),and increase ~3H-TdR incorporation into bone mar- row cells.The data suggest that FLCM contains a CSF synergistic activity.