摘要
The effect of 1,25-dihydroxyvitamin D<sub>3</sub> [1,25（OH）<sub>2</sub>D<sub>3</sub>] and13-cis-retinoic acid（RA）on the proliferation of a novel human megakaryoblasticleukemia cell line（HIMeg）was investigated.At the concentration of 10<sup>9</sup> 10<sup>6</sup>mol/L,1,25（OH）<sub>2</sub>D<sub>3</sub> and RA showed significant inhibition of the proliferation of themegakaryoblastic leukemic cells,which was demonstrated by the count of survival cells,incorporation of<sup>3</sup>H-TdR and<sup>3</sup>H-UR,and cloning efficiency in dose-dependent and time-dependent manners.The results can further explain the mechanism of differentiation-inducing agents and the effect of 1 ,25（OH）<sub>2</sub>D<sub>3</sub> on myelofibrosis.It is possible for1,25（OH）<sub>2</sub>D<sub>3</sub> and RA to be used to treat malignant megakaryocytic diseases.
The effect of 1,25-dihydroxyvitamin D_3 [1,25(OH)_2D_3] and 13-cis-retinoic acid(RA)on the proliferation of a novel human megakaryoblastic leukemia cell line(HIMeg)was investigated.At the concentration of 10~9 10~6 mol/L,1,25(OH)_2D_3 and RA showed significant inhibition of the proliferation of the megakaryoblastic leukemic cells,which was demonstrated by the count of survival cells, incorporation of^3H-TdR and^3H-UR,and cloning efficiency in dose-dependent and time- dependent manners.The results can further explain the mechanism of differentiation- inducing agents and the effect of 1 ,25(OH)_2D_3 on myelofibrosis.It is possible for 1,25(OH)_2D_3 and RA to be used to treat malignant megakaryocytic diseases.
