应用抗PwMJ-SAg单克隆抗体诊断早期和肺外型卫氏并殖吸虫病人循环抗原的研究

STUDIES ON THE DETECTION OF CIRCULATING ANTIGENS IN THE EARLY STAGE OR THE NONPULMONARY TYPE OF PARAGONIMASIS WESTERMANI BY USING MONOCLONAL ANTIBODIES AGAINST PwMJ-SAg

在8个抗PwMJ-SAg单克隆抗体,IB_1和IB_4可检测出35例早期卫氏并殖吸虫病、15例肺外型卫氏并殖吸虫病等患者血清循环抗原,阳性率达100％;与25份正常人血清无交叉反应;与15例华枝睾吸虫病、16例姜片虫病、15例日本血吸虫病、16倒马来丝虫病和10例肺结核病等患者血清均无交叉反应,但与15例斯氏狸殖吸虫病中8例患者血清有交叉反应,交叉反应率为53.3％。因此,单克隆抗体IP_1和IB_4可作为诊断早期和肺外型卫氏并殖吸虫病的适宜试剂,并对斯氏狸殖吸虫病亦有一定的诊断参考价值。

The result regarding the application of McAbs against PwMJ-SAg in the detection of the circulating antigens(CAg) in sera from the patients (early stage and nonpulmonary type) with paragonimiasis westermani was reported in this paper. CAg in the sera from 35 patients in the early stage and 15 nonpulmonary type of patients was 100% positive by using McAb IB1 and JB4 out of 8 McAbs against PwMJ-SAg. Furthermore, these two McAbs didnot react crossly with the sera from 25 normal subjects, 15 patients with clonorchiasis, 15 patients with fasciolopsiasis, 15 patients with schistosomiasis japonica, 16 patients with Brugian filariasis or 10 patients with pulmonary tuberculosis. However, they crossly reacted with 8 samples of sera from 15 patients (53.3%) with pagumogonimiasis skrjabini. The McAb IB1 and IB4, threfore, are appropriate as the reagents to diagnose paragonimiasis westermani (early stage or nonpulmonary type). Moreover, they are also valuable, to a certain degree, to diagnose pagumogonimiasis skrjabini. The projet was supported by NNSFC Department of parasitology .Anhni Medical University, Hefei