微核形成与细胞周期关系的初步研究 Ⅳ、化学诱变剂诱发人淋巴细胞间期各阶段的微核形成

FRELIMINARY STUDIES ON THE RELATIONSHIP BETWEEN MICRONUCLEUS FORMATION AND CELL CYCLE

本实验应用具有诱变作用的化疗药:噻地哌、长春新碱、乙双吗啉等,体内或体外处理诱发人体外周血淋巴细胞微核,通过控制细胞培养时间,放射性自显影及中期细胞阻滞等方法,定量地分析了细胞间期各阶段的微核率。结果表明,间期各阶段均可有不同程度的微核形成,其中最多的是G_1期,其次是G_2期和G_0期。S期细胞的微核率(MNF)较G_1期有极显著的下降,这提示大部分G_1期的微核细胞不能进入S期,使细胞增殖中止,这可能是抗癌药物杀伤肿瘤细胞的机制之一。

In this paper the authors studied quantitatively the micronucleus formation by chemical mutagens at various phases of interphase in liuman lymphocytes induced by means of control of cell culture intervals, autoradiography and block of meta- phase cells etc. The results show that mutagenic chemotherapy drugs : bimolane (treatment in vitro)and thio tepa etc.(treatment in vivo)can induce micronucleus formation at various phases of interphase in lymphocyte cell cycle.The frepuency of micronucleus (MNF) induced at G1 phase is signifcantly higher than at G0 and G2 phase.The MNF at S phase of cells is significantly lower than at G1. This result suggests that most of micronucleated cells at G1 phase do not enter S phase and micronucleus could be formed rarely at S phase of cells.