含铬酵母的微生物诱变性研究

ON TOXICSTY AND MUTAGENICITY OF CHROMIUM RICH BREWYER'S YEAST

已有报道认为金属铬(主要是六价铬)有诱变性。本文对含铬(三价铬)酵母进行了3种微生物诱变试验。Ames试验中加及不加S9,枯草杆菌重组试验中预冷及不预冷方法对铬酵母3个剂量水平均呈阴性,预冷和不预冷方法的试验结果相近。原噬菌体诱导试验为阴性。SOS显色法对铬酵母进行检测的结果,TA_(1535)/PSK_(1002)菌株为阴性,PQ_(35)菌株为稍有可疑。

Schwarz, 1957 has postulated that trivalent chromium is an active constituent of glucose tolerance factor(GTF). Mertz, 1969 denoted that animals with deficiency cf trivalent chromium could result in damage of glucose tolerence or developed to be diabetes, hyperlipemia and arteriosclerosis. The supplementation of chromium is able to invert or prevent the phenomenon above mentioned. The purpose of present paper is to study the probable toxicity and mutagenicity of chromium rich brewyer's yeast. The result exhibited that the LD50 of chromium yeast of rat and mice are all above 21.5g/kg, catagcrized as non toxic and obtained negative responds to micronucleus test, mouse sperm morphology test,Ames test, Rce-Assay, inductest and chromotest of SOS system. We consider it tobe acceptable to utilize brewyer's yeast as carrier of trivalent chromium to be sourse of chromium supplementation for certain population in deficiency of chromium.