EB病毒转化HLA标准抗原B细胞株的建立及鉴定

ESTABLISHMENT OF B LYMPHOBLASTOID CELL LINES WITH WELL DEFINED HLA PHENOTYPE BY EB-VIRUS TRANSFORMATION

本文报导应用EB病毒转化技术,建立7株人B淋巴母细胞样细胞株,其中4株为HLA标准抗原B细胞株。经免疫荧光染色及生物化学分析鉴定,所有细胞株均表达及合成HLAⅠ类和Ⅱ类抗原,可供HLA抗血清筛选及其它研究应用。

Seven human B-lymphoblastoid cell lines were established by EB-virus transformation. Of them, 4 cells were from family members with well-defined HLA phenotype. Cell surface antigens were analysed by immunofluorescence staining. All of the 7 cell lines expressed surface Ig, and high levels of HLA class Ⅰ and class Ⅱ antigens. They did not express T cell markers,and indicate their B cell origin. Biochemical analysis demonstrated that these B cell lines constantly synthesized HLA class Ⅰ and class Ⅱ antigens. The cell lines can be used in HLA anti-sera screening, identification and other ways.