家兔角膜结膜模型用于痢疾活菌苗免疫效果的研究

Study on the Protective Effect of Dysentery Vaccine with a Model of Keratoconjuntiva in Rabbit

采用家兔角膜结膜模型对依链株口眼痢疾福氏2a 和福氏3a 菌苗进行了免疫效果的研究。结果表明这两型菌苗的完全保护率分别为28.5%和19.1%,部份保护率为47.6%和61.9%,病程下降率为55.0%和47.4%,经与对照组作 Ridit 分析,差异非常显著;综合保护率分别为80.5%和76.5%。试验证明,这种模型是适用的,而且较灵长类动物便宜,操作易于掌握,无早期感染;较豚鼠容易获得和饲养,便于操作和观察,值得研究和推广。

The study on the protective effect of oral dysentery vaccines that made fromsd strain of flexneri 2a and flexneri 3a with rabbit keratoconjuntiva model.The resultsshowed that the complete protective rates were 28.5% and 19.1%;the partial protectiverates were 47.6% and 61.9%;the shortening rates of courseof disease were 55.0% and 47.7%respectively.These results compared to control with Ridit Analysis,u=3.95 to 9.48,P<0.001.Its overall protective rates were 80.5% and 76.5% respectively.The test showed thatthis model was suitable and cheaper and its experimental condition was easier undercontrol than primate.Futhermore it would not have any early infection.It was easier inobtaining and feeding and was more convenient for operation and observation than that ofguirea pig.Therefore,this model seems worthy for further study and popularization.