用免疫脂质体诊治人软骨肉瘤可能性的初步探讨

TENTATIVE EXPLORATION OF POSSIBILITY OF THE TREATMENT OF HUMAN CHONDROSARCOMA BY IMMUNOLIPOSOMEs

本实验用同型双功能试剂联接小单层脂质体(SUV)和兔抗人软骨肉瘤抗体,制成联接率为33％的免疫脂质体,并在脂质体表面标记荧光素进行定位效果的测定。结果表明:该免疫脂质体对软骨肉瘤组织呈阳性反应,对正常的软骨组织及胃癌组织均呈阴性反应,说明该免疫脂质体对软骨肉瘤组织有较好的定位效果,提示了用免疫脂质体诊治人软骨肉瘤的可能性。

In this study, a immunoliposome was prepared by homobifunctional reagentEDCI (1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide)as a crosslinker linkingliposome (smallunilamellar vesicles, SUV) and rabbit antibody against humanchondrosarcoma with a linking rate of 33%. Immunofluorescence stain bymarking fluorescein on liposome surface demonstrated that the immunoliposomeshowed positive to chondrosarooma tissue while negative to those of normalcartilage and gastric carcinoma. This indicates the immunoliposome possessfairly good orientation effect suggesting the possibility of treating chondrosar-coma with immunoliposome.