Von Willebrand因子分布的免疫组织化学研究

IMMUNOHISTOCHEMICAL STUDY ON THE DISTRIBUTION OF von WILLEBRAND FACTOR

本文用抗人von Willebrand因子(vWF)单克隆抗体SZ-29,采用免疫金银法和ABC法,研究了vWF在血管、血小板的分布情况。结果显示,vWF分布于腹主动脉,脐动脉和脐静脉的内皮,内皮基膜区、内皮下层及中膜内区,且vWF特异染色以内皮、内皮基膜区染色最深。免疫金银法显示大部分血小板vwF特异银染呈颗粒状分布,约3—5％血小板整体胞体呈现银染。而免疫电镜观察,静止型血小板vWF分布于α-颗粒内,血小板膜没有或极少结合的vwF;激活型血小板,除α-颗粒外,vWF结合于血小板膜及开放管道系统膜上,在血小板伪足,尤其是伪足末端,常呈现vWF特异染色。

By using immunocolloidal gold-silver technique and ABC technique, an antihuman von Willebrand factor (vWF) monoclonal antibody, SZ-29, was applied to study the distribution of vWF in vessels and platelets. The results demonstrated that vWF distributed in the endothelium, basal membrane, subendothelium and media near internal elastic membrane of abdominal aorta, umbilical artery and umbilical vein. And the densest vWF-specific staining was found in the endothelium and its basal membrane. At the microscopical level, we found that vWF-specific staining was granule--like and scattered in most platelets, but about 3-5% of the platelets were found entirely silver-stained. At the ultrastructural level, we found that vWF was stored in α-granules and there was little platelet membrane-bound vWF in resting platelets. However, in stimulated platelets, plenty of membrane-bound vWF was found on the platelet surface and open canalicular system membrane besides the vWF-specific staining in α-granules. And vWF-specific staining might be found in platelet pseudopodia, especially at their tips,