免疫细胞化学方法观察小鼠胸腺哺育细胞的神经内分泌特性

THE IMMUNOCYTOCHEMICAL OBSERVATION OF NEUROENDOCRINE CHARACTERISTIC IN MICE THYMIC NURSE CELLS

本实验通过免疫金标记技术及间接免疫荧光法、PAP免疫细胞化学方法,对小鼠胸腺哺育细胞(thymic nurse cell,TNC)内精氨酸加压素(arginine vasopressin, AVP)和催产素(oxytocin,OT)进行了标记,观察到TNC胞质内存在对AVP、OT抗血清反应的阳性区域,以TNC胞质周边和其内淋巴细胞(lymphocyte in thymic nurse cell,TNC-L)周围尤为明显。结果表明TNC具有神经内分泌特性,其胞质内的AVP和OT可能对TNC-L的分化发育有一定影响。

The arginine vasopressin (AVP) and oxytocin (OT) in thymic nurse cells (TNCs) of mice were stained by the method of immunogold and indfrect immunofluorescence, PAP immunocytochemical technique. TNCs of mice were shown immunocytochemical reactivity with antfserum of AVP and OT. The posttive areas were much denser near the cells membrane and around TNC-lymphocytes (TNC-Ls). It was implicated that TNCs might serve as neuroendocrine cells and might play a role in TNC-Ls differentiation via secreting AVP and OT.