摘要
用静置培养法已在体外建立了一株人肝癌细胞系,定名 GHC—1.该细胞系来自一位62岁男性病人外科手术切除的标本,至今已传50余代,生长稳定.细胞的形态呈多角形上皮样,染色体数目分布范围较大,主流数集中于54—59之间,为亚三倍体.异种动物接种成瘤(3/3),各种免疫学方法测得细胞和旧培液均含有 AFP.
A human hepatoma cell line designated GHC-1 has been established by stationary culture method in vitro.This line of cells was derived from specimen removed during the surgical opera- lion of a 62-year old male patient and up to now has given a steady growth and has been serially through more than 50 generations.The cells are polygonal epithelial-like in morphology. Chromosome analysis showed a wide variation with a mode around 54-59.This line of cells may be considered hypotripioid as well as tumor formation(3/3)after heterotransplantation.By means of various immunologic methods both the old medium and the cells show they contain AFP
出处
《广州医学院学报》
1990年第3期8-15,共8页
Academic Journal of Guangzhou Medical College
