煤焦沥青烟雾提取物的细胞毒性和遗传毒性研究

STUDIES ON GENOTOXICITY AND CYTOTOXICITY OF THE EXTRACTS FROM COAL TAR PITCH FUMES

采用人外周血淋巴细胞非程序DNA合成(UDS)试验和人胚肺成纤维细胞转化试验,测试了煤焦沥青烟雾提取物(ECTPF)对人体细胞的细胞毒性和遗传毒性。UDS试验结果表明,ECTPF可使淋巴细胞UDS值明显增加,并有剂量一反应关系。引起半数淋巴细胞死亡的浓度(LC50)为33.8μg/ml。细胞转化试验表明,ECTPF能诱发人胚肺成纤维细胞明显的形态学转化,且转化细胞具有部分恶性转化细胞的特性。引起半数人胚肺成纤维细胞生长抑制的浓度为41.3μg/ml。实验结果提示,ECTPF是一种具有细胞毒性和遗传毒性的物质。

Genotoxicity and cytotoxicity of the extracts from coal tar pitch fumes (ECTPF) were studied using human lymphocyte unscheduled DNA synthesis (UDS) test and human embryo lung fibroblastic cell transformation test. The results of UDS test showed that the ECTPF increased UDS value significantly with a does-response relationship. Median lethal concentration (LC59) to human lymphocyte cells is about 33.8μg/ml. The results of human embryo lung fibroblastic cell transformation test showed that the foci of cell morphological transformation(multilayer, criss-crossing foci) appeared 7-10 weeks after treatment, and cell continued to grow in vitro for over 220 days. The transformed cells had a high growth rate and ability of growth on semi-solid agar medium and agglutination by cancanavalin A. Median lethal concentration to human embryo lung fibroblastic cell is about 41.3μg/ml. The results of our study showed that the ECTPF is a matter with genotoxicity and cytoxicity.