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应用荧光微球技术进行免疫检测研究 被引量：2

Study on Immunoassay by Fluorescent Microsphere-Based Technology
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摘要 以聚苯乙烯羧基荧光微球为固相载体,将人免疫球蛋白G(IgG)和猪血红蛋白(Hb)分别与微球偶联,应用Luminex-100TM荧光微球检测系统检测抗体IgG和抗原Hb与荧光微球的偶联效率。结果表明,采用100μg.mL-1抗体或抗原浓度可以获得较高的偶联效率,且检测快速灵敏。应用荧光微球进行抗原抗体免疫检测可行。 Human immunoglobin G（IgG） and swine hemoglobin（Hb） were coupled to the carboxylated polystyrene fluorescent microsphere used as solid carrier,and Luminex-100TM system was used to detect the coupling efficiency between fluorescent microsphere and IgG or Hb.Results showed that,the maximal coupling efficiency could be achieved by using 100 μg·mL-1 antibody or antigen,and the detection was fast and sensitive.It is feasible to apply fluorescent microsphere technology to immunodetection.
作者 张燕玲 陈超 杨慧 薛小平
机构地区 西北工业大学生命学院 国家微检测系统工程技术研究中心
出处 《化学与生物工程》 CAS 2011年第11期84-86,共3页 Chemistry & Bioengineering
基金 国家自然科学基金资助项目(31000067) 西北工业大学基础科研基金资助项目(JC200921)
关键词 荧光微球 抗体 抗原 免疫检测 fluorescent microsphere antibody antigen immunodetection
分类号 O657.3 [理学—分析化学] R446.61 [医药卫生—诊断学]
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参考文献12

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共引文献8

同被引文献31

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引证文献2

二级引证文献18

化学与生物工程
2011年 第11期

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