酶联免疫法快速检测贝类中腹泻性贝类毒素

Rapid Detection of Diarrhetic Shellfish Poisons by ELISA Method

应用美国ABRAXIS腹泻性贝类毒素(DSP)试剂盒对广西钦州和防城港海域近江牡蛎各20个样品、北海海域文蛤20个样品进行了ELISA法DSP检测。全部检测过程在2 h内完成,检测限为0.1 ppb,灵敏度为1.7 ppb。ELISA法检测DSP操作方便、成本低,检测限和灵敏度均满足要求。ELISA法有望作为理想的DSP快速筛选分析方法之一。

The United States ABRAXIS diarrhetic shellfish poisons（DSP） determination kit was applied to determine DSP in 20 oyster samples from Qinzhou seawater,20 oyster samples from Fangchenggang seawater and 20 clam samples from North Sea seawater.All the detection was completed in 2 h with the detection limit of 0.1 ppb and the sensitivity of 1.7 ppb.ELISA method was convenient with low cost,while its detection limit and sensitivity were sufficient to meet the requirements,so it was expected to playing an important role in rapid detection of aquatic products,in surveys of shellfish poisons in breeding areas,and in quality control of shellfish in market as an ideal rapid screening method for DSP.