摘要
目的研究用分化诱导剂全反式维甲酸(ATRA)培养HL-60细胞株后GPI-PLD的表达变化,以初步探讨该酶在全反式维甲酸(ATRA)作用下的变化及其与白血病细胞生长增殖的关系。方法用ATRA处理HL-60细胞后,以胎盘碱性磷酸酶为底物,定量检测GPI-PLD活性;RT-PCR法确定GPI-PLD mRNA表达水平;MTT检测细胞增殖;流式细胞仪检测细胞周期。结果加入全反式维甲酸培养后,HL-60细胞株中GPI-PLD表达量和活性增加。流式细胞仪检测显示细胞阻滞在G2/M期;MTT实验检测显示ATRA处理后HL-60细胞株增殖生长受到抑制。结论加ATRA处理后HL-60细胞株增殖生长受到抑制,可能与GPI-PLD表达量显著增加有关。
Objective To investigate the expression of glycosylphosphatidyl inositol specific phospholipase D（GPI-PLD） in leukemia cell HL-60 after ATRA added to the culture medium,to find out the relative function of GPI-PLD in the course of leukemia generating and developing,as well as to make out the influence of ATRA on GPI-PLD. Methods RT-PCR was used to detect the expression level of GPI-PLD mRNA in HL-60 cell line after ATRA added to the culture medium.GPI-PLD activities were analyzed quantitatively by TX-114 partition with GPI anchored placental alkaline phosphatase（PLAP） as a substrate.MTT was used to detect the proliferation in each group,and the cell circle of leukemia cell induced by ATRA was determined by the flow cytometer. Results After ATRA added to the culture medium,the mRNA expression and activities of GPI-PLD in HL-60 cell line were significantly increased.The proliferative capacity was significantly decreased and the cell cycle was arrested in G2/M phase. Conclusions The ATRA can induce expression of GPI-PLD and GPI-PLD probably has contact with leukemias generating and developing.
出处
《实用预防医学》
CAS
2011年第11期2055-2057,共3页
Practical Preventive Medicine
